Back to Search Start Over

Molecularly Imprinted Polymer-Amyloid Fibril-Based Electrochemical Biosensor for Ultrasensitive Detection of Tryptophan

Authors :
Ibrar Alam
Benchaporn Lertanantawong
Thana Sutthibutpong
Primana Punnakitikashem
Piyapong Asanithi
Source :
Biosensors, Vol 12, Iss 5, p 291 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

A tryptophan (Trp) sensor was investigated based on electrochemical impedance spectroscopy (EIS) of a molecularly imprinted polymer on a lysozyme amyloid fibril (MIP-AF). The MIP-AF was composed of aniline as a monomer chemically polymerized in the presence of a Trp template molecule onto the AF surface. After extracting the template molecule, the MIP-AF had cavities with a high affinity for the Trp molecules. The obtained MIP-AF demonstrated rapid Trp adsorption and substantial binding capacity (50 µM mg−1). Trp determination was studied using non-Faradaic EIS by drop drying the MIP-AF on the working electrode of a screen-printed electrode. The MIP-AF provided a large linear range (10 pM–80 µM), a low detection limit (8 pM), and high selectivity for Trp determination. Furthermore, the proposed method also indicates that the MIP-AF can be used to determine Trp in real samples such as milk and cancer cell media.

Details

Language :
English
ISSN :
20796374
Volume :
12
Issue :
5
Database :
Directory of Open Access Journals
Journal :
Biosensors
Publication Type :
Academic Journal
Accession number :
edsdoj.f40f60384d5746729809956146342c4a
Document Type :
article
Full Text :
https://doi.org/10.3390/bios12050291