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Evaluation of mismatch-repair and microsatellite-instability status in a Chinese colorectal cancer Cohort

Authors :
Peng Zhang
Anqi Wang
Ce Bian
Jing Zhang
Caifeng Jiang
Haiyang Zhou
Source :
Asian Journal of Surgery, Vol 47, Iss 2, Pp 959-967 (2024)
Publication Year :
2024
Publisher :
Elsevier, 2024.

Abstract

Background: Immunohistochemistry (IHC) and traditional polymerase chain reaction (PCR) are the methods of choice in clinical practice to identify the mismatch repair (MMR) and microsatellite instability (MSI) status in colorectal cancer (CRC). In some previous researches, the concordance rate between two methods was different and discordance existed in about 1 %–9.7 %. Methods: We retrospectively reviewed 406 patients received surgical CRC resections and tests of both MMR IHC and MSI PCR from January 2019 to April 2022 in Shanghai Changzheng Hospital. The incidence of deficient mismatch repair (dMMR) or microsatellite instability-high (MSI-H) CRCs, the concordance rate between two methods, and the reasons for discordant results were evaluated with clinicopathological data, immunochemical staining, whole-exome sequencing, and MLH1 methylation analysis. Results: Among 406 patients, the incidence of MSI-H CRCs was 7.88 %. Nearly a quarter of the cases under reexamination of IHC was initial misinterpreted. Besides, the concordance rate between MMR IHC and MSI PCR was 99.26 % (401 of 404) and the Kappa value was 0.945 (p

Details

Language :
English
ISSN :
10159584
Volume :
47
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Asian Journal of Surgery
Publication Type :
Academic Journal
Accession number :
edsdoj.f6405be88f9c4375acd27de03aaae471
Document Type :
article
Full Text :
https://doi.org/10.1016/j.asjsur.2023.12.176