Back to Search Start Over

Quantitative Proteomic Analysis Reveals Key Proteins Involved in Testicular Development of Yaks

Authors :
Yongfu La
Xiaoming Ma
Pengjia Bao
Min Chu
Ping Yan
Xian Guo
Chunnian Liang
Source :
International Journal of Molecular Sciences, Vol 25, Iss 15, p 8433 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Male reproductive health is largely determined already in the early development of the testis. Although much work has been carried out to study the mechanisms of testicular development and spermatogenesis, there was previously no information on the differences in the protein composition of yak testicles during early development. In this study, the protein profiles in the testicles of 6- (M6), 18- (M18), and 30-month-old (M30) yaks were comparatively analyzed using TMT proteomics. A total of 5521 proteins were identified, with 13, 1295, and 1397 differentially expressed proteins (DEPs) in 30- vs. 18-, 18- vs. 6-, and 30- vs. 6-month-old testes, respectively. Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that DEPs were mainly involved in signaling pathways related to testicular development and spermatogenesis, including the MAPK, PI3K–Akt, Wnt, mTOR, TGF-β, and AMPK signaling pathways. Furthermore, we also identified eight potential proteins (TEX101, PDCL2, SYCP2, SYCP3, COL1A1, COL1A2, ADAM10, and ATF1) that may be related to the testicular development and spermatogenesis of yaks. This study may provide new insights into the molecular mechanisms of the testicular development and spermatogenesis of yaks.

Details

Language :
English
ISSN :
14220067 and 16616596
Volume :
25
Issue :
15
Database :
Directory of Open Access Journals
Journal :
International Journal of Molecular Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.f72b993404815bf684accd6a397e4
Document Type :
article
Full Text :
https://doi.org/10.3390/ijms25158433