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Protocol for generating human induced neural progenitor cells from immobilized adult peripheral blood
- Source :
- STAR Protocols, Vol 2, Iss 1, Pp 100346- (2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- Summary: Generating induced neural stem/progenitor cells (iNPCs) from somatic cells for medical applications has remained challenging. Here, we describe a reliable protocol to make human iNPCs from a small volume of immobilized adult peripheral blood by direct reprogramming. We have verified that the integration-free human iNPCs can efficiently differentiate into mature neurons in mouse brain upon transplantation and display capacities to functionally replace the damaged neurons, suggesting their potential as donor cells in developing replacement medicine for neurodegenerative diseases.For complete details on the use and execution of this protocol, please refer to Zhang et al. (2019).
- Subjects :
- Cell isolation
Neuroscience
Stem cells
Cell differentiation
Science (General)
Q1-390
Subjects
Details
- Language :
- English
- ISSN :
- 26661667
- Volume :
- 2
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- STAR Protocols
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.f861a540c60a42deb25a8bac86d761c1
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.xpro.2021.100346