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Investigation of Carriers of Salmonella and Other Hydrogen Sulphide-Positive Bacteria in the Digestive Content of Fish from the Atlantic Area of Macaronesia: A Comparative Study of Identification by API Gallery and MALDI-TOF MS

Authors :
Inmaculada Rosario Medina
Marco Antonio Suárez Benítez
María del Mar Ojeda-Vargas
Kiara Gallo
Daniel Padilla Castillo
Miguel Batista-Arteaga
Soraya Déniz Suárez
Esther Licia Díaz Rodríguez
Begoña Acosta-Hernández
Source :
Animals, Vol 14, Iss 22, p 3247 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Salmonella spp. are known pathogens in fish, with their presence potentially resulting from the contamination of the aquatic environment or improper handling. Accurate bacterial identification is crucial across various fields, including medicine, microbiology, and the food industry, and thus a range of techniques are available for this purpose. In this study, Salmonella spp. and other hydrogen sulphide-positive bacteria were investigated in the digestive contents of fish destined for consumption from the Atlantic area of Macaronesia. Two identification techniques were compared: the traditional API method and the MALDI-TOF MS technique. For the identification of Salmonella spp. carriers, 59 samples were processed following ISO 6579–1:2017. A total of 47 strains of Gram-negative bacilli were obtained. No Salmonella spp. isolates were detected. The most frequent genus was Enterobacter (76.50%), followed by Shewanella (10.63%). The MALDI-TOF MS technique showed a high concordance with the API technique, with 72.34% concordance at the species level. Both techniques demonstrated a high degree of concordance in the identification of Enterobacter cloacae, with 87.23% genus-level concordance and 12.76% non-concordant identifications. This study highlights the limitations of the API technique and the speed and precision of MALDI-TOF MS. The identified bacteria could pose a health risk to humans.

Details

Language :
English
ISSN :
20762615
Volume :
14
Issue :
22
Database :
Directory of Open Access Journals
Journal :
Animals
Publication Type :
Academic Journal
Accession number :
edsdoj.f8e4143129b4aacaf9ccc405c46f099
Document Type :
article
Full Text :
https://doi.org/10.3390/ani14223247