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Validation and application of a blood screening method for SARS-CoV-2 nucleic acid
- Source :
- Zhongguo shuxue zazhi, Vol 35, Iss 1, Pp 10-13 (2022)
- Publication Year :
- 2022
- Publisher :
- Institute of Blood Transfusion of Chinese Academy of Medical Sciences, 2022.
-
Abstract
- Objective To evaluate the performance and clinical application of a high-throughput nucleic acid blood screening detection system for SARS-CoV-2, so as to provide basis and technical means for its application in detection of plasma from recovered COVID-19 patients. Methods The SARS-CoV-2 nucleic acid was detected by real-time fluorescence quantitative PCR, and the sensitivity, precision, anti-interference and other parameters were evaluated. Blood donor samples collected during COVID-19 epidemic period were screened using the detection system to evaluate its applicability. Results The detection limits of gene N and ORF 1ab were 3.98 copies/mL and 9.38 copies/mL, respectively. The CV of high and low concentration samples were both less than 5%. Hemoglobin at 500 mg/dL and triglyceride at 3g/dL had little effect on the results. The detection system can effectively prevent carryover, thus avoiding false positive results. The SARS-CoV-2 nucleic acid blood screening was carried out in a total of 39 306 blood samples, and all samples were negative. Conclusion The established method can meet the needs of SARS-CoV-2 nucleic acid screening therefore ensure the safe application of plasma from recovered COVID-19 patients.
Details
- Language :
- Chinese
- ISSN :
- 1004549X, 1004549x, and 96377291
- Volume :
- 35
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Zhongguo shuxue zazhi
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.f9637729140c488496e40fcfe2943737
- Document Type :
- article
- Full Text :
- https://doi.org/10.13303/j.cjbt.issn.1004-549x.2022.01.003&lang=en