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Inhibitory effect of AK-7 mediates by apoptosis, increases DNA fragmentation and caspase-3 activity in human glioblastoma multiforme cells

Authors :
Ebru Güçlü
İlknur Çınar Ayan
Hasibe Vural
Source :
Bangladesh Journal of Pharmacology, Vol 17, Iss 2 (2022)
Publication Year :
2022
Publisher :
Bangladesh Pharmacological Society, 2022.

Abstract

Sirtuins (SIRTs) which are nicotinamide adenine dinucleotide (NAD+) dependent class III histondeacetylases have a controversial role in cancer. In this study, the effect of pharmacological inhibition of AK-7, a SIRT2 inhibitor, was investigated in U87 glioblastoma multiforme cells. The cytotoxic effect of AK-7 was evaluated by XTT analysis. After AK-7 treatment, colony forming capacity of cells was determined and apoptosis was evaluated. The expression levels of apoptosis-related genes were determined by qRT-PCR. According to the results, AK-7 inhibited cell proliferation in a dose- and time-dependent manner. After AK-7 treatment, the colony forming capacity of U87 cells was suppressed. And, AK-7 increased apoptosis rate, DNA fragmentation, and caspase-3 activity. According to qRT-PCR, a significant increase was observed in expression levels of apoptosis-related genes. This study revealed that AK-7 inhibits cell proliferation and induces apoptosis in glioblastoma multiforme cells and SIRT2 inhibition can be evaluated as a therapeutic approach in glioblastoma multiforme.

Details

Language :
English
ISSN :
19910088
Volume :
17
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Bangladesh Journal of Pharmacology
Publication Type :
Academic Journal
Accession number :
edsdoj.f971ed82f9254d9088b919d5237b9f35
Document Type :
article
Full Text :
https://doi.org/10.3329/bjp.v17i2.59809