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Antimicrobial Effects of 5-Aminolevulinic Acid Mediated Photodynamic Therapy against Pathogenic Bacteria

Authors :
Pil Seung Kwon
Source :
Korean Journal of Clinical Laboratory Science, Vol 54, Iss 4, Pp 273-278 (2022)
Publication Year :
2022
Publisher :
The Korean Society for Clinical Laboratory Science, 2022.

Abstract

This study evaluates the improved effect of photodynamic therapy (PDT) by subjecting pathogenic bacteria to a combination of 630 nm light-emitting diode (LED) and 5-aminolevulinic acid (ALA). Bacterial suspensions of 1.5×104 cells/mL were diluted and exposed to ALA concentrations of 10, 5, 2.5, 1.25, and 0.625 mg/mL, incubated for 30 minutes, followed by irradiation with 630 nm LED (18 J/cm2). The non-irradiated P. aeruginosa group and the group administered only LED light averaged 415 and 245 colonies, respectively. Conversely, the PDT group showed an average of 109, 225, 297, and 285 colonies at concentrations of 10, 5, 2.5, and 1.25 mg/mL of ALA. Evaluating the effect on E. faecalis revealed an average of 8,750 and 8,000 colonies in the group that did not receive the control photosensitizer and the group exposed to light alone, respectively. However, an average of 0, 2350, 4825, and 7475 colonies at concentrations of 5, 2.5, 1.25, and 0.625 mg/mL ALA were determined for the PDT groups. In conclusion, better inhibitory effects were observed for E. faecalis than for P. aeruginosa. Moreover, our results validate the possibility of improved PDT efficacy using a combination of ALA and 630 nm LED.

Details

Language :
English, Korean
ISSN :
17383544
Volume :
54
Issue :
4
Database :
Directory of Open Access Journals
Journal :
Korean Journal of Clinical Laboratory Science
Publication Type :
Academic Journal
Accession number :
edsdoj.f990d02f3ab4f8b9f45a33b029e436a
Document Type :
article
Full Text :
https://doi.org/10.15324/kjcls.2022.54.4.273