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Functional Characterization of PoEP1 in Regulating the Flowering Stage of Tree Peony

Authors :
Yang Lei
Jingshan Gao
Yuying Li
Chengwei Song
Qi Guo
Lili Guo
Xiaogai Hou
Source :
Plants, Vol 13, Iss 12, p 1642 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

The tree peony, a traditional flower in China, has a short and concentrated flowering period, restricting the development of the tree peony industry. To explore the molecular mechanism of tree peony flowering-stage regulation, PoEP1, which regulated the flowering period, was identified and cloned based on the transcriptome and degradome data of the early-flowering mutant Paeonia ostii ‘Fengdan’ (MU) and Paeonia ostii ‘Fengdan’ (FD). Through bioinformatics analysis, expression pattern analysis, and transgene function verification, the role of PoEP1 in the regulation of tree peony flowering was explored. The open-reading frame of PoEP1 is 1161 bp, encoding 386 amino acids, containing two conserved domains. PoEP1 was homologous to the EP1 of other species. Subcellular localization results showed that the protein was localized in the cell wall and that PoEP1 expression was highest in the initial decay stage of the tree peony. The overexpression of PoEP1 in transgenic plants advanced and shortened the flowering time, indicating that PoEP1 overexpression promotes flowering and senescence and shorten the flowering time of plants. The results of this study provide a theoretical basis for exploring the role of PoEP1 in the regulation of tree peony flowering.

Details

Language :
English
ISSN :
13121642 and 22237747
Volume :
13
Issue :
12
Database :
Directory of Open Access Journals
Journal :
Plants
Publication Type :
Academic Journal
Accession number :
edsdoj.f9c2fb46dcb88a5b0a791f9a73
Document Type :
article
Full Text :
https://doi.org/10.3390/plants13121642