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Punicalagin Inhibits African Swine Fever Virus Replication by Targeting Early Viral Stages and Modulating Inflammatory Pathways

Authors :
Renhao Geng
Dan Yin
Yingnan Liu
Hui Lv
Xiaoyu Zhou
Chunhui Bao
Lang Gong
Hongxia Shao
Kun Qian
Hongjun Chen
Aijian Qin
Source :
Veterinary Sciences, Vol 11, Iss 9, p 440 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

African swine fever (ASF), caused by the African swine fever virus (ASFV), has resulted in significant losses in the global pig industry. Considering the absence of effective vaccines, developing drugs against ASFV may be a crucial strategy for its prevention and control in the future. In this study, punicalagin, a polyphenolic substance extracted from pomegranate peel, was found to significantly inhibit ASFV replication in MA-104, PK-15, WSL, and 3D4/21 cells by screening an antiviral compound library containing 536 compounds. Time-of-addition studies demonstrated that punicalagin acted on early viral replication stages, impinging on viral attachment and internalization. Meanwhile, punicalagin could directly inactivate the virus according to virucidal assay. RT-qPCR and Western blot results indicated that punicalagin modulated the NF-κB/STAT3/NLRP3 inflammasome signaling pathway and reduced the levels of inflammatory mediators induced by ASFV. In conclusion, this study reveals the anti-ASFV activity of punicalagin and the mechanism of action, which may have great potential for developing effective drugs against ASFV.

Details

Language :
English
ISSN :
23067381
Volume :
11
Issue :
9
Database :
Directory of Open Access Journals
Journal :
Veterinary Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.fa0e05ded0840d9820baef3b4d1cb4c
Document Type :
article
Full Text :
https://doi.org/10.3390/vetsci11090440