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Modulation of apoptosis by V protein mumps virus
- Source :
- Virology Journal, Vol 8, Iss 1, p 224 (2011)
- Publication Year :
- 2011
- Publisher :
- BMC, 2011.
-
Abstract
- Abstract Background The Urabe AM9 vaccine strain of mumps virus contains two variants of V protein: VWT (of HN-A1081 viral population) and VGly (of HN-G1081). The V protein is a promoting factor of viral replication by blocking the IFN antiviral pathway. Findings We studied the relationship between V protein variants and IFN-α2b-induced apoptosis. V proteins decrease activation of the extrinsic IFN-α2b-induced apoptotic pathway monitored by the caspase 8 activity, being the effect greater with the VWT protein. Both V proteins decrease the activity of caspase 9 of the intrinsic apoptotic pathway. In a system without IFN, the VWT and VGly proteins expression promotes activation of caspases 3 and 7. However, when the cellular system was stimulated with IFN-α, this activity decreased partially. TUNEL assay shows that for treatment with IFN-α and ibuprofen of cervical adenocarcinoma cells there is nuclear DNA fragmentation but the V protein expression reduces this process. Conclusions The reduction in the levels of caspases and DNA fragmentation, suggesting that V protein, particularly VWT protein of Urabe AM9 vaccine strain, modulates apoptosis. In addition, the VWT protein shows a protective role for cell proliferation in the presence of antiproliferative signals.
- Subjects :
- Infectious and parasitic diseases
RC109-216
Subjects
Details
- Language :
- English
- ISSN :
- 1743422X
- Volume :
- 8
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Virology Journal
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.fadd94b9638742c8b3391444bce7358d
- Document Type :
- article
- Full Text :
- https://doi.org/10.1186/1743-422X-8-224