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Development and validation of RT-LAMP for detecting yellow fever virus in non-human primates samples from Brazil

Development and validation of RT-LAMP for detecting yellow fever virus in non-human primates samples from Brazil

Authors :
Sabrina F. Cardoso
Andre Akira Gonzaga Yoshikawa
Iara Carolini Pinheiro
Lucilene Wildner Granella
Dinair Couto-Lima
Maycon Sebastião Alberto Santos Neves
Daniel Santos Mansur
André N. Pitaluga
Luísa D. P. Rona
Source :
Scientific Reports, Vol 14, Iss 1, Pp 1-10 (2024)
Publication Year :
2024
Publisher :
Nature Portfolio, 2024.

Abstract

Abstract Monitoring yellow fever in non-human primates (NHPs) is an early warning system for sylvatic yellow fever outbreaks, aiding in preventing human cases. However, current diagnostic tests for this disease, primarily relying on RT-qPCR, are complex and costly. Therefore, there is a critical need for simpler and more cost-effective methods to detect yellow fever virus (YFV) infection in NHPs, enabling early identification of viral circulation. In this study, an RT-LAMP assay for detecting YFV in NHP samples was developed and validated. Two sets of RT-LAMP primers targeting the YFV NS5 and E genes were designed and tested together with a third primer set to the NS1 locus using NHP tissue samples from Southern Brazil. The results were visualized by colorimetry and compared to the RT-qPCR test. Standardization and validation of the RT-LAMP assay demonstrated 100% sensitivity and specificity compared to RT-qPCR, with a detection limit of 12 PFU/mL. Additionally, the cross-reactivity test with other flaviviruses confirmed a specificity of 100%. Our newly developed RT-LAMP diagnostic test for YFV in NHP samples will significantly contribute to yellow fever monitoring efforts, providing a simpler and more accessible method for viral early detection. This advancement holds promise for enhancing surveillance and ultimately preventing the spread of yellow fever.

Details

Language :
English
ISSN :
20452322
Volume :
14
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Scientific Reports
Publication Type :
Academic Journal
Accession number :
edsdoj.fbd2a3026ca44fba99465468ba4b5599
Document Type :
article
Full Text :
https://doi.org/10.1038/s41598-024-74020-4