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Establishment of a rat model of chronic thoracolumbar cord compression with a flat plastic screw
- Source :
- Neural Regeneration Research, Vol 11, Iss 6, Pp 963-970 (2016)
- Publication Year :
- 2016
- Publisher :
- Wolters Kluwer Medknow Publications, 2016.
-
Abstract
- Previous studies of animal models of chronic mechanical compression of the spinal cord have mainly focused on cervical and thoracic lesions, but few studies have investigated thoracolumbar injury. The specific pathophysiological mechanism of chronic thoracolumbar cord injury has not yet been elucidated. The purpose of this study was to improve animal models of chronic thoracolumbar cord compression using the progressive screw. A custom-designed flat plastic screw was implanted in the spinal cord between thoracic vertebrae 12 and lumbar 1 of rats. The screw was tightened one complete turn (0.5 mm) every 7 days for 4 weeks to create different levels of chronic spinal cord compression. Following insertion of the screw, there was a significant decline in motor function of the hind limbs, and severe stenosis of micro-computed tomography parameters in the spinal cord. Cortical somatosensory evoked potential amplitudes were reduced remarkably, and latencies were prolonged at 30 minutes after surgery. The loss of motor neurons in the gray matter was marked. Demyelination and cavitation were observed in the white matter. An appropriate rat model of chronic thoracolumbar cord compression was successfully created using the progressive screw compression method, which simulated spinal cord compression injury.
- Subjects :
- nerve regeneration
spinal cord compression
chronic spinal cord injury
cortical somatosensory evoked potential
electrophysiology
micro-computed tomography
animal model
behavioral evaluation
Basso
Beattie and Bresnahan score
histopathology
neural regeneration
Neurology. Diseases of the nervous system
RC346-429
Subjects
Details
- Language :
- English
- ISSN :
- 16735374
- Volume :
- 11
- Issue :
- 6
- Database :
- Directory of Open Access Journals
- Journal :
- Neural Regeneration Research
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.fbfc9ac7a154f85bc4a86c493d4b794
- Document Type :
- article
- Full Text :
- https://doi.org/10.4103/1673-5374.184496