Back to Search Start Over

Quantitative Rapid Test for Detection and Monitoring of Active Pulmonary Tuberculosis in Nonhuman Primates

Authors :
Zijie Zhou
Anouk van Hooij
Richard Vervenne
Claudia C. Sombroek
Elisa M. Tjon Kon Fat
Tom H. M. Ottenhoff
Paul L. A. M. Corstjens
Frank Verreck
Annemieke Geluk
Source :
Biology, Vol 10, Iss 12, p 1260 (2021)
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

Nonhuman primates (NHPs) are relevant models to study the pathogenesis of tuberculosis (TB) and evaluate the potential of TB therapies, but rapid tools allowing diagnosis of active pulmonary TB in NHPs are lacking. This study investigates whether low complexity lateral flow assays utilizing upconverting reporter particles (UCP-LFAs) developed for rapid detection of human serum proteins can be applied to detect and monitor active pulmonary TB in NHPs. UCP-LFAs were used to assess serum proteins levels and changes in relation to the MTB challenge dosage, lung pathology, treatment, and disease outcome in experimentally MTB-infected macaques. Serum levels of SAA1, IP-10, and IL-6 showed a significant increase after MTB infection in rhesus macaques and correlated with disease severity as determined by pathology scoring. Moreover, these biomarkers could sensitively detect the reduction of bacterial levels in the lungs of macaques due to BCG vaccination or drug treatment. Quantitative measurements by rapid UCP-LFAs specific for SAA1, IP-10, and IL-6 in serum can be utilized to detect active progressive pulmonary TB in macaques. The UCP-LFAs thus offer a low-cost, convenient, and minimally invasive diagnostic tool that can be applied in studies on TB vaccine and drug development involving macaques.

Details

Language :
English
ISSN :
20797737
Volume :
10
Issue :
12
Database :
Directory of Open Access Journals
Journal :
Biology
Publication Type :
Academic Journal
Accession number :
edsdoj.fcbe3c23ac7465393b01836dfc19347
Document Type :
article
Full Text :
https://doi.org/10.3390/biology10121260