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Isolation, Characterization, and Genome Insight of Pseudomonas jordanii: A Novel Endophyte Enhancing Durum Wheat (Triticum turgidum ssp. durum) Growth under Salinity Stress

Authors :
Randa N. Albdaiwi
Tareq Alhindi
Hala Khyami-Horani
Shireen Hasan
Hesham M. Al-Younes
Source :
Applied Sciences, Vol 14, Iss 15, p 6681 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Pseudomonas jordanii strain G34 is a moderately halophilic endophytic bacterium isolated from the root tissue of durum wheat plants growing in the saline environment of the Jordan Valley’s Ghor Sweimeh region. Microscopic and biochemical analyses of P. jordanii strain G34 revealed that it is a Gram-negative, non-motile rod. It also exhibits capsule formation, catalase and oxidase positive reactions, indole positivity, citrate utilization, and non-glucose fermenting capability. Pseudomonas jordanii strain G34 showed growth-promoting effects on durum wheat seedlings grown under severe salinity stress conditions up to a 200 mM NaCl concentration. The draft genome of P. jordanii strain G34 comprises 5,142,528 base pairs (bp) and possesses a G + C content of 64.0%. It contains 57 RNA coding genes and is predicted to encode a total of 4675 protein-coding genes. Putative genes linked to various aspects of the bacterial endophyte lifestyle were identified including ion transport, motility, secretion, adhesion, delivery systems, and plant cell wall modification. Performing a comprehensive phylogenomic analysis identified P. jordanii as a new species, with its closest relative being P. argentinensis LMG 22563, sharing only around 40.2% digital DNA-DNA hybridization identity. Pseudomonas jordanii strain G34 holds great potential for future use as a biofertilizer in saline environments.

Details

Language :
English
ISSN :
20763417
Volume :
14
Issue :
15
Database :
Directory of Open Access Journals
Journal :
Applied Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.fe206764c87e4988a6b30149165a86d7
Document Type :
article
Full Text :
https://doi.org/10.3390/app14156681