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Ecofriendly Validated RP-HPTLC Method for Simultaneous Determination of the Bioactive Sesquiterpene Coumarins Feselol and Samarcandin in Five Ferula Species Using Green Solvents

Authors :
Maged S. Abdel-Kader
Mohammed H. Alqarni
Sura Baykan
Bintug Oztürk
Mohammad Ayman A. Salkini
Hasan S. Yusufoglu
Prawez Alam
Ahmed I. Foudah
Source :
Separations, Vol 9, Iss 8, p 206 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

An environmentally friendly unreported rapid and simple reverse-phase high-performance thin-layer chromatography (RP-HPTLC) has been designed for the simultaneous determination of bioactive sesquiterpene coumarins feselol and samarcandin in the methanol extract of five Ferula species. The method was developed using glass plates coated with RP-18 silica gel 60 F254S and a green solvent system of ethanol–water mixture (8:2 v/v) as mobile phase. After development, the plates were quantified densitometrically at 254 for feselol and samarcandin. Feselol and samarcandin peaks from methanol extract of five Ferula species were identified by comparing their single band at Rf = 0.43 ± 0.02 and Rf = 0.60 ± 0.01, respectively. Valid linear relationships between the peak areas and concentrations of feselol and samarcandin in the range of 1000–7000 ng/band respectively were obtained. The method was subjected to the validation criteria of the international conference on harmonization (ICH) for precision, accuracy, and robustness. The new method provides an analytical tool to enumerate the therapeutic doses of feselol and samarcandin in herbal formulations and/or crude drugs. The obtained results indicated that F. drudeana was the richest species in the more active samarcandin, with 0.573% w/w, while F. duranii had the largest quantity of the less active feselol, 0.813% w/w. F. drudeana was superior to the other species in the sum of the two active compounds, 1.4552% w/w, and was consequently expected to be the most active aphrodisiac among the five studied species.

Details

Language :
English
ISSN :
22978739
Volume :
9
Issue :
8
Database :
Directory of Open Access Journals
Journal :
Separations
Publication Type :
Academic Journal
Accession number :
edsdoj.ff45ba3a2bb40b0a20fe02caf604e65
Document Type :
article
Full Text :
https://doi.org/10.3390/separations9080206