Expression of TNF, IL1B, and NOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti -Porphyromonas gingivalis [version 2; peer review: 1 approved with reservations]

Authors :: Endang Winiati Bachtiar
Citra F. Putri
Retno D. Soejoedono
Boy M. Bachtiar
Author Affiliations :: <relatesTo>1</relatesTo>Oral Biology and Oral Science Research Center, Faculty of Dentistry Universitas Indonesia, Jakarta, DKI, 10430, Indonesia<br /><relatesTo>2</relatesTo>Department of Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia
Source :: F1000Research. 9:1499
Publication Year :: 2021
Publisher :: London, UK: F1000 Research Limited, 2021.
Abstract: Porphyromonas gingivalis has virulence factors such as gingipain and lipopolysaccharide, causing bacteremia to reach the brain and activate neuroinflammatory release cytokines. This study analyzed the effect of the co-culture of neuron cells with P. gingivalis coated with anti -P. gingivalis antibodies against cytokines produced by neuron cells. The gene expressions of the TNF, IL1B, NOS2 in neurons was evaluated using RT-qPCR. The results showed that P. gingivalis coated with anti -P. gingivalis antibody before co-culture with neuron cells could decrease the gene expression of TNF, IL1B, and NOS2 of neuron cells.

Subjects :: Brief Report
Articles
Porphyromonas gingivalis
Blocking Antibody
Neuroinflammation
TNF-α
IL-1β
iNOS

ISSN :: 20461402
Volume :: 9
Database :: F1000Research
Journal :: F1000Research
Notes :: Revised Amendments from Version 1 Here is the summary of the specific changes made from version 1 to version 2 of our manuscript. 1. In first paragraph of page 2, we add: “P gingivalis reside in a structured community of biofilm attached to surfaces embedded in the extracellular matrix which they produce themselves and they are difficult to eradicate due to their resistance to antimicrobials and the body's defense mechanisms 10. The passive immunization approach using polyclonal antibodies to inhibit P gingivalis adhesion to the periodontium tissue is a strategy to prevent biofilm formation and periodontium tissue damage which can lead to deeper tissue invasion so that P gingivalis can enter the systemic circulation.” Reference citation number “10” has been added. 2. In paragraph 3 of page 5 line 3, we insert: “pooled.” 3. In page 5: “morphology’ has been deleted. 4. The references order number was changed as consequences of added one reference above, , [version 2; peer review: 1 approved with reservations]
Publication Type :: Academic Journal
Accession number :: edsfor.10.12688.f1000research.26749.2
Document Type :: brief-report
Full Text :: https://doi.org/10.12688/f1000research.26749.2

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