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Hypoxic Preconditioning Promotes Survival of Human Adipose Derived Mesenchymal Stem Cell [version 4; peer review: 3 approved]
- Source :
- F1000Research. 10:843
- Publication Year :
- 2024
- Publisher :
- London, UK: F1000 Research Limited, 2024.
-
Abstract
- Background: Contributing factors for improved survival of human adipocytes mesenchymal stem cells (h-AMSCs) cultured through hypoxia preconditioning, in example apoptosis inhibition involving BCL2 and HSP27 expression, trigger signal expression (VEGF), SCF expression, OCT-4 expression, and CD44+ expression. The objective if this study was to explain the mechanism and role of hypoxic preconditioning and the optimal duration of hypoxic preconditioning exposure to improve survival of h-AMSCs. Methods: An experimental laboratory explorative study ( in vitro) with hypoxic preconditioning in h-AMSCs cultures. This research was conducted through four stages. First, isolation of h-AMSCs culture from adipose tissue of patients. Second, the characterization of h-AMSCs from adipose tissue by phenotype (flowcytometry) through CD44+, CD90+ and CD45-expression before being pre-conditioned for hypoxic treatment. Third, the hypoxic preconditioning in h-AMSCs culture ( in vitro) was performed with an oxygen concentration of 1% for 24, 48 and 72 hours. Fourth, observation of survival from h-AMSCs culture was tested on the role of CD44+, VEGF, SCF, OCT-4, BCL2, HSP27 with Flowcytometry and apoptotic inhibition by Tunnel Assay method. Results: The result of regression test showed that time difference had an effect on VEGF expression ( pβ=-0.482) and hypoxia condition also influenced VEGF expression ( pβ=0.774). The result of path analysis showed that SCF had effect on OCT-4 expression ( pβ=0.985). The regression test results showed that time effects on HSP27 expression ( pβ=0.398) and hypoxia precondition also affects HSP27 expression ( pβ=0.847). Pathway analysis showed that BCL2 expression inhibited apoptosis ( p=0.030; β=-0.442) and HSP27 expression also inhibited apoptosis ( pβ=-0.487). Conclusion: Hypoxic preconditioning of h-AMSC culture has proven to increase the expression of VEGF, SCF, OCT-4, and BCL2 and HSP27. This study demonstrated and explained the existence of a new mechanism of increased h-AMSC survival in cultures with hypoxic preconditioning (O2 1%) via VEGF, SCF, OCT-4, BCL2, and HSP 27.
- Subjects :
- Research Article
Articles
apoptosis
h-AMSCs
BCL-2
HSP27
SCF
VEGF expression
Subjects
Details
- ISSN :
- 20461402
- Volume :
- 10
- Database :
- F1000Research
- Journal :
- F1000Research
- Notes :
- Revised Amendments from Version 3 - Minor change and simplification of the title with deleted "via expression of prosurvival and proangiogenic biomarkers”. - Minor change of the conclusion statement according to the results: “From this study, it can be concluded that the hypoxic preconditioning of h-AMSC culture has proven to increase the expression of VEGF, SCF, OCT-4, and BCL2 and HSP27. This study demonstrated and explained the existence of a new mechanism of increased h-AMSC survival in cultures with hypoxic preconditioning (O 2 1%) via VEGF, SCF, OCT-4, BCL2, and HSP 27.” - Figure 10 : Path analysis had been moved from the conclusion section into the last subsection of the results section., , [version 4; peer review: 3 approved]
- Publication Type :
- Academic Journal
- Accession number :
- edsfor.10.12688.f1000research.55351.4
- Document Type :
- research-article
- Full Text :
- https://doi.org/10.12688/f1000research.55351.4