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Reaction of Pseudomonas fluorescens Kynureninase with Beta-Benzoyl-L-alanine: detection of a new reaction intermediate and a change in rate-determinig step
- Source :
- Biochemistry. March 23, 2004, Vol. 43 Issue 11, 3230-3237
- Publication Year :
- 2004
-
Abstract
- The pre-steady-state kinetics of the reaction of Beta-benzoyl-L-alanine was investigated by rapid scanning stopped-flow spectrophotometry. The spectra show the formation of a quinonoid intermediate and also support the formation of benzoate as the rate-determining step.
Details
- Language :
- English
- ISSN :
- 00062960
- Volume :
- 43
- Issue :
- 11
- Database :
- Gale General OneFile
- Journal :
- Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.121828520