IspH protein of Escherichia coli: Studies on iron-sulfur cluster implementation and catalysis

Authors :: Grawert, Tobias
Kaiser, Johannes
Zepeck, Ferdinand
Laupitz, Ralf
Hecht, Stefan
Amslinger, Sabine
Schramek, Nicholas
Schleicher, Erik
Weber, Stefan
Halsbeck, Martin
Buchner, Johannes
Reider, Christoph
Arigoni, Duilio
Bacher, Adelbert
Eisenreich, Wolfgang
Rohdich, Felix
Source :: Journal of the American Chemical Society. Oct 13, 2004, Vol. 126 Issue 40, p12847, 9 p.
Publication Year :: 2004
Abstract: A study to show that the recombinant expression of the protein in functional form can be greatly enhanced by coexpression of genes from the isc operon, which are required for the assembly of iron-sulfur clusters, is presented. The EPR experiments as well as optical absorbance show the presence of [3Fe-4S]+ cluster in IspH protein.

Subjects :: Proteins -- Chemical properties
Iron compounds -- Chemical properties
Catalysis -- Methods
Escherichia coli -- Research
Chemistry

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