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Substrate specificity and kinetic mechanism of the Sir2 family of NAD+-dependent histone/protein deacetylases
- Source :
- Biochemistry. August 3, 2004, Vol. 43 Issue 30, p9877, 11 p.
- Publication Year :
- 2004
-
Abstract
- The substrate specificity of the yeast Silent information regulator 2 (Sir2), the yeast HST2 and the human SIRT2 homologues toward various monoacetylated histone H3 and H4 peptides are analysed to study the basic kinetic mechanism and individual chemical steps of the Sir2 reaction. Steady-state kinetic analysis reveals that ySir2, HST2 and SIRT2 exhibit varying catalytic efficiencies and display a preference among the monoacetylated peptide substrates.
Details
- Language :
- English
- ISSN :
- 00062960
- Volume :
- 43
- Issue :
- 30
- Database :
- Gale General OneFile
- Journal :
- Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.124844317