Synthesis, Physicochemical and biochemical studies of 1',2'.-oxetane constrained adenosine and guanosine modified oligonucleotides, and their comparison with those of the corresponding cytidine and thymidine analogues

The synthesis of the oxetane-A and oxetane-G nuclesides and their incorporations into antisense oligonucleotides(AONs) and their structural and antisensence properties are compared with those of the T and C modified AONs.Oxetane-A and -G modified AONs showed their target affinity with complimentary RNA, and the thermodynamic parameters to be identical or very close to the native AON/RNA duplex.