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Surface plasmon fluorescence immunoassay of free prostate-specific antigen in human plasma at the femtomolar level
- Source :
- Analytical Chemistry. Nov 15, 2004, Vol. 76 Issue 22, p6765, 6 p.
- Publication Year :
- 2004
-
Abstract
- This study describes the development of a surface plasmon fluorescence spectroscopy (SPFS)-based sandwich immunoassay, for the detection of free prostate-specific antigen (f-PSA). The commercial CM5 chip (Biacore) was integrated into the SPFS-based assay making use of its three-dimensionally extended dextran architecture, which offers a large protein retention capacity and alleviates the metal-induced fluorescence quenching. The performance of the f-PSA assay was investigated in buffer and in human plasma, respectively. In the human plasma, the limit of detection of f-PSA was estimated to be ~80 fM for 40 min of contact time, which adequately meets clinical requirements.
- Subjects :
- Spectrum analysis -- Research
Chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 00032700
- Volume :
- 76
- Issue :
- 22
- Database :
- Gale General OneFile
- Journal :
- Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.125645917