Engineering a hybrid sugar biosynthetic pathway: production of L-rhamnose and its implication on dihydrostreptose biosynthesis

The Strptomyces venezuelae strains containing selected genes from the L-dihydrostreptose pathway are constructed and the biosynthetic implications obtained for the dihydrostreptose pathway are reported. The versatility of the glycosyltransferase on substrate selection highlights its potential as a catalyst in the construction of new macrolides carrying a broad range of modified sugars.