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In vitro reconstruction of an NADPH-dependent superoxide reduction pathway from Pyrococcus furiosus
- Source :
- Applied and Environmental Microbiology. March, 2005, Vol. 71 Issue 3, p1522, 9 p.
- Publication Year :
- 2005
-
Abstract
- The main aim of the study is to obtain the recombinant form of Pyrococcus furiosus NAD(P)H:rubredoxin oxidoreductase (NROR), to reconstitute an in vitro recombinant superoxide reduction pathway and to show that NADPH can serve as an electron source for superoxide reduction to peroxide. It is demonstrated that the complete pathway from NAD(P)H to reduction of superoxide reductase (SOR) via NROR and rubredoxin can be reconstituted in an in vitro recombinant system.
Details
- Language :
- English
- ISSN :
- 00992240
- Volume :
- 71
- Issue :
- 3
- Database :
- Gale General OneFile
- Journal :
- Applied and Environmental Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.134076710