Biosynthesis of TDP-L-mycarose: The specificity of a single enzyme governs the outcome of the pathway

Sequence analysis of genes found in the biosynthetic gene clusters has resulted in tentative assignments for the encoded proteins and their putative functions in the biosynthetic pathways. Critical evidence assigning the roles of the key enzymes involved in the TDP-L-mycarose biosynthesis and complete the reconstitution of the entire mycarose pathway in vitro is provided.