1H, 13C, and 15N chemical shift assignments for the unligated enzyme and analysis of chemical shift changes that accompany formation of the nuclease-thymidine 3'5'-biphosphate-calcium ternary complex

Accurate 1H, 13C and 15N chemical shifts were assigned for staphylococcal nuclease H124L. The chemical shits of unligated nuclease H124L were then compared with those of the nuclease-thymidine 3',5'-biphosphate-calcium (H124L.pdTp.Ca2+) ternary complex. The results show that backbone 15N, 1H(super N), 1H(super alpha) and 13C(super alpha) resonancesin certain sections of the amino acid sequence show large shifts upon binding pdTp and Ca2+.