(super 13)C-carrier DNA shortens the incubation time needed to detect benzoate-utilizing denitrifying bacteria by stable-isotope probing

Authors :: Gallagher, E.
McGuinness, L.
Phelps, C.
Young, L.Y.
Kerkhot, L.J.
Source :: Applied and Environmental Microbiology. Sept, 2005, Vol. 71 Issue 9, 5192-5196
Publication Year :: 2005
Abstract: Genetic signatures from denitrifying bacteria capable of benzoate utilization in coastal sediments were discerned using DNA-stable isotope probing (SIP) and (super 13)C-carrier DNA for the physical separation of (super 13)C-DNA. Results suggest that temporal analysis using SIP can be used to illustrate the initial biodegrader in a bacterial population and to document the cross-feeding microbial community.

Subjects :: DNA -- Research
Denitrification -- Research
Isotopes -- Research
Biodegradation -- Research
Biological sciences

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