Development and validation of a fluorescence polarization-based competitive peptide-binding assay for HLA-A*0201--A new tool for epitope discovery

A novel state-of-the-art biochemical HLA-A*0201 assay, which is comprised of technologically advanced cutting edge reagents is developed and validated to better facilitate epitope discovery. Results obtained demonstrated significant correlation among assay procedures, suggesting that the application of fluorescence polarization in combination with recombinant sHLA molecules is highly advantageous for the accurate assessment of peptide binding.