Adhesion to fibronectin stimulates inositol lipid synthesis and enhances PDGF-induced inositol lipid breakdown

The role of inositol lipids in mediating some of the effects of extracellular matrix on cellular form and functions was investigated. The study protocol involved the measurement of the effects of adhesion to fibronectin (FN) on the lipid phosphatidylinositol bisphosphate (PIP2) and its hydrolysis products diacylglycerol and inositol triphosphate. PIP2 and its hydrolysis products have functions in cytoskeletal regulation and as second messengers, respectively. The reults showed that adhesion to FN stimulates inositol lipid synthesis and increases PDGF-induced inositol lipid breakdown.