A two-stage one-pot enzymatic synthesis of TDP-L-mycarose from thymidine and glucose-1-phosphate

Several experiments are conducted to reveal a new two-stage one-pot method for the enzymatic biosynthesis of TDP-L-mycarose from the readily available precursors, glucose-1-phosphate and thymidine. The process described demonstrates the use of enzymes in the synthesis of several unstable TDP-sugar derivatives and hence can be widely used for the identification of more such enzymes in addition to the synthesis of other unusual sugars.