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Evidence for two active sites in the spliceosome provided by stereochemistry of pre-mRNA splicing
- Source :
- Nature. Sept 23, 1993, Vol. 365 Issue 6444, p364, 5 p.
- Publication Year :
- 1993
-
Abstract
- The exchange of a 3'-5'- for 2'-5' bond in the first step of lariat formation and the exchange of one 3'-5' phosphodiester for another in the second step of exon ligation are the two different reactions for subtracting the introns from nuclear precursors to messenger RNAs (pre-mRNAs) by the spliceosome. A study conducted to detect the stereochemical course for each step, utilizing the splicing substrate containing phosphothioate, showed that both steps occur as a single 'in-line' Sn2 nucleophilic substitution reaction, similar to the mechanism of group I self splicing introns. The reaction sites for second step of spliceosomal processing is similar to that of group I self splicing introns.
Details
- ISSN :
- 00280836
- Volume :
- 365
- Issue :
- 6444
- Database :
- Gale General OneFile
- Journal :
- Nature
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.14567332