HIV-1 reverse transcriptase mutants resistant to nonnucleoside reverse transcriptase inhibitors do not adversely affect DNA synthesis: Pre-steady-state and steady-state kinetic studies

The polymerase function of 3 clinically occurring nonnucleoside reverse transcriptase inhibitor (NNRTI)-resistant RTs in greater detail, under both pre-steady-state and steady-state conditions is evaluated. Results indicate that the close proximity of the NNRTI-resistance mutations to the polymerase active site does not affect the interactions of the enzyme with the incoming nucleotide or the primer-template sufficiently to affect polymerization.