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A novel single-photon counting technique applied to highly sensitive measurement of [[[Ca.sup.2+]].sub.i] transient in human aortic smooth muscle cells
- Source :
- Journal of Biomechanical Engineering. Oct, 2006, Vol. 128 Issue 5, p777, 5 p.
- Publication Year :
- 2006
-
Abstract
- This study demonstrates that aequorin, a luminescent natural dye, is useful for vascular cell intracellular [Ca.sup.2+] concentration ([[[Ca.sup.2+]].sub.i]) determination. A new single-photon counting technique was developed to resolve the effects of fluid flow shear stress on [[[Ca.sup.2+]].sub.i] in human aortic smooth muscle cells (HASMCs). Confluent HASMCs were grown on petri dishes loaded with aequorin. Then the dishes were placed in a luminometer chamber after the physiological level of shear stress was applied to the HASMC surfaces. The chamber was housed inside a highly sensitive photomultiplier tube. It detected ultraweak photon emission in response to the [[[Ca.sup.2+]].sub.i] transient. In the presence of 2.0 mM extracellular [Ca.sup.2+], a shear stress of 12 dyn/[cm.sup.2], applied for 60 s to the top surface of the HASMC monolayer, elicited a sharp increase in [[[Ca.sup.2+]].sub.i]. [DOI: 10.1115/1.2264396] Keywords: ultraweak photon-counting, smooth muscle cells, shear stress, intracellular [Ca.sup.2+], biophoton
Details
- Language :
- English
- ISSN :
- 01480731
- Volume :
- 128
- Issue :
- 5
- Database :
- Gale General OneFile
- Journal :
- Journal of Biomechanical Engineering
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.153413697