Entropy of water in the hydration layer of major and minor grooves of DNA

The entropy of water molecules is calculated in the major and minor grooves of a B-DNA duplex and in the bulk using a statistical mechanical scheme developed by Goddard and co-workers. The calculations for the water under equilibrium conditions appropriately shows that the undoubted entropy gain time T is canceled by the energetic effects in the process of transferal of water from the grooves of DNA to the bulk.