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Gold nanoparticle based FRET assay for the detection of DNA cleavage

Authors :
Darbha, Gopala Krishna
Ray, Paresh Chandra
Fortner, Angela
Source :
Journal of Physical Chemistry B. Oct 26, 2006, Vol. 110 Issue 42, 20745-20748
Publication Year :
2006

Abstract

Gold nanoparticle based fluorescence resonance energy transfer (FRET) assay is reported to monitor the cleavage of DNA by nucleases. Florescence signal enhancement is detected by a factor of 120 after the cleavage reaction in the presence of S1 nuclease.

Details

Language :
English
ISSN :
15206106
Volume :
110
Issue :
42
Database :
Gale General OneFile
Journal :
Journal of Physical Chemistry B
Publication Type :
Academic Journal
Accession number :
edsgcl.154670587