A structural basis for the chiral preferences of lipases

Authors :: Cygler, Miroslaw
Grochulski, Pawel
Kazlauskas, Romas J.
Schrag, Joseph D.
Bouthillier, Francois
Rubin, Byron
Serreqi, Alessio N.
Gupta, Ajay K.
Source :: Journal of the American Chemical Society. April 20, 1994, Vol. 116 Issue 8, p3180, 7 p.
Publication Year :: 1994
Abstract: X-ray crystallography was used to determine the mechanism by which lipase from Candida rugosa differentiates between enantiomers of menthol. This mechanism may elucidate the structural features in lipases that are associated with enantiorecognition. Phosphonates were covalently linked to the active site serene to imitate the transition state for menthyl ester hydrolysis.