Immunoquantitative real-time PCR for detection and quantification of Staphylococcus aureus enterotoxin B in foods

A real-time immunoqunatitative PCR (iqPCR) method for detection of Staphylococcus aureus enterotoxin B (SEB) was developed and evaluated using both pure cultures and foods. IqPCR was not inhibited by any of the foods tested and was able to detect SEB present in these foods and no cross reactivity with SE other than SEB was observed.