Role for the PP2A/B56[delta] phosphatase in regulating 14-3-3 release from Cdc25 to control mitosis

The DNA-responsive checkpoints are found to activate PP2A/B56[delta] phosphatase complexes to dephosphorylate Cdc25 at a site distinct from Ser287 (T138), the phosphorylation of which is required for 14-3-3 release. The results have identified PP2A/B56[delta] as a vital checkpoint effector and have described a mechanism for showing that a critical component of checkpoint regulation lies with the PP2A-mediated dephosphorylation of T128 and consequent release of 14-3-3.