The Role of a Phenylalanine Ammonia Lyase Gene during Cassava Bacterial Blight and Cassava Bacterial Necrosis

Byline: LF Pereira (1), PH Goodwin (2), L Erickson (3) Keywords: Keywords: Gene expression, Manihot esculenta, Phenylalanine ammonia lyase, Xanthomonas axonopodis pv. manihotis, Xanthomonas cassavae Abstract: ). PAL enzyme activity and MEPAL mRNA levels were measured in leaves of cultivar MCOL 22 following mechanical wounding or inoculation with 2 bacterial pathogens. MCOL 22 is resistant to Xanthomonas cassavae, which causes cassava bacterial necrosis and susceptible to X. axonopodis pv. manihotis, which causes cassava bacterial blight. PAL enzyme activity in the resistant interaction was significantly higher than in the susceptible interaction or the control. Similarly, MEPAL transcripts were detected in leaves during the resistant interaction, but not during the susceptible reaction. RFLP analysis with MEPAL revealed a divergence between South American and African/Asian cultivars and showed promise for MEPAL as a marker for resistance to Xanthomonads. Author Affiliation: (1) Area de Ecofisiologia, IAPAR, Londrina, Pr Brasil, BR (2) Department of Environmental Biology, University of Guelph, Guelph Ontario NIG 2WI, Canada, CA (3) Department of Plant Agriculture, University of Guelph, Guelph Ontario NIG 2WI, Canada, CA Article note: Received 25 September 1998/ Accepted in revised form 4 January 1999