Cytosolic (Ca(super 2+)) measurements in endothelium of rabbit cardiac valves using imaging fluorescence microscopy

Imaging fluorescence microscopic study of the endothelium of rabbit cardiac valves reveals cytosolic endothelial calcium ion measurements in an intact monolayer without being subjected to enzyme treatment. Individual cells with intact intercellular links and functional muscarinic acetylcholine receptor presence enhance comprehension of endothelial cell signalling. Pathophysiological states such as mechanical disruption which occurs in angioplasty and catheterization mechanisms are studied using this method.