Improved method for recovery of mRNA from aquatic samples and its application to detection of mer expression

Authors :: Jeffrey, Wade H.
Nazaret, Sylvie
Haven, Robin von
Source :: Applied and Environmental Microbiology. June, 1994, Vol. 60 Issue 6, p1814, 8 p.
Publication Year :: 1994
Abstract: Boiling sodium dodecyl sulfate-diethylpyrocarbonate lysis combined with acid-guanidinium extraction recovers undamaged, contaminating DNA free, mRNA target sequences from environment and pure cultures. The mRNA detects mercuric reductase A transcripts in the microbial population of mercury-contaminated pond water and enhances the study of mer gene characteristics.

Subjects :: Messenger RNA -- Research
Chromosome mapping -- Analysis
Genetic transcription -- Analysis
Biological sciences

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