Mechanism-based inactivation of benzoylformate decarboxylase, A thiamin diphosphate-dependent enzyme

Authors :: Bera, Asim K.
Polovnikova, Lena S.
Roestamadji, Juliatek
Widlanski, Theodore S.
Kenyon, Geroge L.
McLeish, Michael J.
Hasson, Miriam S.
Source :: Journal of the American Chemical Society. April 11, 2007, Vol. 129 Issue 14, p4120, 2 p.
Publication Year :: 2007
Abstract: A report is presented on the discovery of a mechanism-based inhibitor of benzoylformate decarboxylase (BFD) that covalently modifies the enzyme via active site phosphorylation, a type of mechanism-based inactivation. The report highlights that benzoylphosphonate acts as mechanism-based inhibitor BFD, with inactivation resulting from the phosphoryl-action of Ser26.

Subjects :: Decarboxylases -- Chemical properties
Decarboxylases -- Structure
Binding sites (Biochemistry) -- Structure
Phosphorylation -- Research
Vitamin B1 -- Chemical properties
Chemistry

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