DNA alkylation by pyrrole-imidazole seco-CBI conjugates with an indole linker: Sequence-specific DNA alkylation with 10-base-pair recognition through heterodimer formation

The sequence-specific DNA alkylation by conjugates with an indole linker is examined in the absence or presence of partner Py-Im polyamine. The results have shown that alkylation has proceeded through heterodimer formations, indicating that this is a general way to expand the recognition sequence for DNA alkylation by N-methylpyrrole (Py)-N-methylimidazole (Im) polyamides and 1-(chloromethyl)-5-hydroxy-1,2-dihydro-3H-benz[e]indole (seco-CBI) conjugates.