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Utility of CE--MS data in protein identification
- Source :
- Analytical Chemistry. May 15, 2007, Vol. 79 Issue 10, p3850, 6 p.
- Publication Year :
- 2007
-
Abstract
- A new method for displaying CE-MALDI-MS data for proteolytic digests is described. This data display mode yields distinct charge-based trends for plots of mass-to-charge (m/z) versus CE migration time. These trends arise owing to the in-solution charge state of the peptides, and this interpretation was confirmed by using empirical peptide electromigration models and peptide standards as charge-state markers. These charge-state specific trends exhibit analytical utility by providing additional chemical information about the peptides, which increases the confidence level of peptide identification and provides a rapid means for screening for posttranslationally modified peptides.
Details
- Language :
- English
- ISSN :
- 00032700
- Volume :
- 79
- Issue :
- 10
- Database :
- Gale General OneFile
- Journal :
- Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.164871213