Nitric oxide attenuates leukocyte-endothelial interaction via P-selectin in splanchnic ischemia-reperfusion

Intravital microscopic studies show that splanchnic artery ischemia and reperfusion (SAO/R) in rats increases the leukocyte-endothelial interaction in intestinal venules. Addition of exogenous NO donor S-nitroso-N-acetylpenicillamine (SNAP) decreases the leukocyte-endothelial interaction and plasma proteolysis. SNAP pretreatment decreases the SAO/R-induced P-selectin expression. Immunohistochemical data show that altering the leukocyte-endothelial interaction by NO proceeds through the endothelial adhesion molecule P-selectin.