Oxidant stress decreases Na+/H+ antiport activity in bovine pulmonary artery endothelial cells

Analysis of the effects of oxidant stress on the Na+/H+ antiport activity in monolayers exposed in the oxidant, tert-butyl hydroperoxide (t-BOOH), shows that oxidant stress attenuates Na+/H+ antiport activity in bovine pulmonary artery endothelial cells. Na+/H+ antiport regulates intracellular pH in these cells and t-BOOH decreases the rate of recovery from acidosis. The intracellular glutathione in these cells enable the maintenance of Na+/H+ antiport functions.