Extensive sugar modification improves triple helix forming oligonucleotide activity in vitro but reduces activity in vivo

Authors :: Alam, Md. Rowshon
Sasaki, Shigeki
Seidman, Michael M.
Miller, Paul S.
Su-Ting Liu
Puri, Nitin
Cuenoud, Bernard
Ji-Lan Liu
Majumdar, Alokes
Thazhathveetil, Arun Kalliat
Source :: Biochemistry. Sept 4, 2007, Vol. 46 Issue 35, 10222-10233
Publication Year :: 2007
Abstract: Triple helix forming oligonucleotides (TFOs) are developed for gene targeting and their influence on bioactivity of the modifications is examined. The enhancement of TFO bioactivity has required chemical modifications that improve interaction with the specific targets while retaining selectivity against mismatched sequences.

Subjects :: DNA microarrays -- Analysis
Nucleotide sequence -- Analysis
Biological sciences
Chemistry

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