2'-Deoxynucleoside dithiophosphates: synthesis and biological studies

A novel technique enables the synthesis of deoxyadenosine 5'- and 3'-dithiophosphate and thymidine 5'- and 3'-dithiophosphate. The technique couples deoxynucleoside H-phosphonodithioates and 9-fluorenemethanol in an oxidative environment, which yields 9-fluorenemethyl phosphoradithioates. Deprotection of 9-fluorenemethyl phosphoradithioates by ammonium hydroxide, followed by purification yields the dithiophosphates. In vitro studies on these compounds as inhibitor of enzyme activity for potential therapeutic use show no inhibitory effects.