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Directed evolution of AraC for improved compatibility of arabinose- and lactose-inducible promoters

Authors :
Sung Kuk Lee
Chou, Howard H.
Pfleger, Brian F.
Newman, Jack D.
Yoshikuni, Yasuo
Keasling, Jay D.
Source :
Applied and Environmental Microbiology. Sept, 2007, Vol. 73 Issue 17-18, 5711-5715
Publication Year :
2007

Abstract

The directed evolution of AraC to build an arabinose-inducible system, which is more compatible with (isopropyl-[beta]-D-1-thiogalactopyranoside) induction of a lactose-inducible system is presented.

Subjects

Subjects :
Aldoses -- Structure
DNA-ligand interactions -- Research
Polymerase chain reaction -- Analysis
Escherichia coli -- Physiological aspects
Escherichia coli -- Research
Biological sciences

Details

Language :
English
ISSN :
00992240
Volume :
73
Issue :
17-18
Database :
Gale General OneFile
Journal :
Applied and Environmental Microbiology
Publication Type :
Academic Journal
Accession number :
edsgcl.169890229

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